Expression and clinical significance of CD44V5 and CD44V6 in resectable colorectal cancer

J Cancer Res Clin Oncol. 2004 Nov;130(11):679-86. doi: 10.1007/s00432-004-0596-1. Epub 2004 Aug 6.

Abstract

Purpose: This study was conducted to evaluate the prognostic significance of CD44v5 and CD44v6 in resectable colorectal cancer.

Materials and methods: Membranous CD44v5 and CD44v6 levels were measured by an immunoenzymatic assay in tumors and surrounding mucosal samples obtained from 105 patients with resectable colorectal carcinomas.

Results: There were no significant differences of CD44v5 levels between tumors [median: 3.2 (range: 0.9-83.5) ng/mg protein) and surrounding mucosal samples (3 (3-146.2) ng/mg protein]. However, tumor samples showed significantly higher CD44v6 levels [19.5 (2.2-562.9) ng/mg protein] than mucosal samples [5 (5-230) ng/mg protein] (P=0.0001). Patients with higher CD44v5 or CD44v6 content in tumor samples had a considerably shorter relapse-free survival (P<0.05, for both). Patients with a higher CD44v6 content also had a shorter relapse-free and overall survival in the multivariate analysis (P<0.05).

Conclusion: The results of this study suggest a role of CD44v5 and CD44v6 in colorectal cancer progression. Membranous CD44v levels in primary tumors, measured by immunoenzymatic assay, may contribute to a more precise prognostic estimation in patients with resectable colorectal cancer.

Publication types

  • Clinical Trial

MeSH terms

  • Aged
  • Carcinoma / immunology*
  • Carcinoma / pathology
  • Carcinoma / surgery*
  • Cell Adhesion
  • Colorectal Neoplasms / immunology*
  • Colorectal Neoplasms / pathology
  • Colorectal Neoplasms / surgery*
  • Disease-Free Survival
  • Female
  • Flow Cytometry
  • Glycoproteins / biosynthesis*
  • Glycoproteins / immunology
  • Humans
  • Hyaluronan Receptors / biosynthesis*
  • Hyaluronan Receptors / immunology
  • Immunoassay
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Predictive Value of Tests
  • Prognosis
  • Prospective Studies
  • Sensitivity and Specificity

Substances

  • CD44v5 antigen
  • CD44v6 antigen
  • Glycoproteins
  • Hyaluronan Receptors