Effects of hyperbaric oxygen therapy on caustic esophageal injury in rats

J Pediatr Surg. 2004 Aug;39(8):1188-93. doi: 10.1016/j.jpedsurg.2004.04.012.

Abstract

Background/purpose: Caustic esophageal burn is a serious problem in pediatric surgery. Even though many clinical and experimental studies had been performed, the complication rate could not be reduced to a satisfying level. In this study, the authors evaluated the effects of hyperbaric oxygen (HBO) therapy in caustic esophageal burn in rats.

Methods: Rats were divided into 4 groups, and caustic burn at the distal esophagus was created by applying 50% NaOH for 3 minutes in all groups. The first and third groups did not receive HBO therapy. HBO therapy was applied to the second group for 2 days and to the fourth group for 28 days. To evaluate the effects of short-term HBO therapy, the first 2 groups were compared for ulceration, inflammation, and submucosal vascular thrombosis after 2 days. The third and fourth groups were compared for the long-term effects of HBO therapy. Rats in these groups were killed after 28 days and compared for the collagen content, weight, and mortality rate.

Results: In the second group, which received 2 days of HBO therapy, ulcer depth and vascular thrombosis were significantly lower than these in the first group (P =.022 and P =.020, respectively). The fourth group, which received 4 weeks of HBO therapy, had a significantly reduced mortality rate, weight loss, and collagen score and hydroxyproline level if compared with the third group (P =.035; P =.016; P =.028; and P =.033, respectively).

Conclusions: These results indicate that HBO therapy is useful in caustic esophageal burn both in short-term and long-term use.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Burns, Chemical / therapy*
  • Collagen / analysis
  • Esophageal Spasm, Diffuse / etiology
  • Esophageal Spasm, Diffuse / therapy
  • Esophagitis / chemically induced
  • Esophagitis / therapy*
  • Esophagus / injuries*
  • Esophagus / pathology
  • Fibrosis
  • Hydroxyproline / analysis
  • Hyperbaric Oxygenation*
  • Rats
  • Rats, Wistar
  • Sodium Hydroxide / toxicity
  • Thrombosis / etiology
  • Thrombosis / therapy
  • Time Factors
  • Ulcer / etiology
  • Ulcer / therapy
  • Weight Loss

Substances

  • Sodium Hydroxide
  • Collagen
  • Hydroxyproline