Abstract
Expression of TNF-alpha gene in the retinal pigment epithelial (RPE) cells was studied by using polymerase chain reaction (PCR). PCR for human TNF-alpha gene using complementary DNA (cDNA) of control RNA prepared from non-stimulated RPE cells failed to show expression of TNF-alpha gene. However, PCR by using cDNA prepared from interleukin-1 beta (IL-1 beta)-stimulated RPE cells revealed expression of the gene, suggesting in vivo production of TNF-alpha from RPE cells in response to IL-1 beta.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cells, Cultured
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Cloning, Molecular
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DNA / chemistry
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DNA / genetics
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Gene Expression*
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Humans
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Interleukin-1 / pharmacology
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Kinetics
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Molecular Sequence Data
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Pigment Epithelium of Eye / metabolism*
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Platelet-Derived Growth Factor / pharmacology
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Polymerase Chain Reaction
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Tetradecanoylphorbol Acetate / pharmacology
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Thrombin / pharmacology
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Transforming Growth Factor beta / pharmacology
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Tumor Necrosis Factor-alpha / genetics*
Substances
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Interleukin-1
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Platelet-Derived Growth Factor
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
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DNA
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Thrombin
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Tetradecanoylphorbol Acetate