FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion

Geeske Zanen; Haike Antelmann; Helga Westers; Michael Hecker; Jan Maarten van Dijl; Wim J Quax

doi:10.1128/JB.186.17.5956-5960.2004

FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion

J Bacteriol. 2004 Sep;186(17):5956-60. doi: 10.1128/JB.186.17.5956-5960.2004.

Authors

Geeske Zanen¹, Haike Antelmann, Helga Westers, Michael Hecker, Jan Maarten van Dijl, Wim J Quax

Affiliation

¹ Department of Molecular Bacteriology, University of Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.

Abstract

Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and FtsY. Although flhF is located in the fla/che operon, B. subtilis 168 flhF mutant cells assemble flagella and are motile.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics
Bacillus subtilis / metabolism*
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Flagella / genetics
Flagella / physiology
Genes, Bacterial
Monomeric GTP-Binding Proteins / genetics*
Monomeric GTP-Binding Proteins / metabolism
Movement
Mutagenesis, Insertional
Mutation
Operon
Protein Transport
Proteome / analysis
Proteome / isolation & purification
Signal Recognition Particle / genetics
Signal Recognition Particle / physiology*

Substances

Bacterial Proteins
Proteome
Signal Recognition Particle
flhF protein, Bacteria
Monomeric GTP-Binding Proteins