Characterization of binding of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase to Porphyromonas gingivalis major fimbriae

Kazuhiko Maeda; Hideki Nagata; Masae Kuboniwa; Kosuke Kataoka; Nobuko Nishida; Muneo Tanaka; Satoshi Shizukuishi

doi:10.1128/IAI.72.9.5475-5477.2004

Characterization of binding of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase to Porphyromonas gingivalis major fimbriae

Infect Immun. 2004 Sep;72(9):5475-7. doi: 10.1128/IAI.72.9.5475-5477.2004.

Authors

Kazuhiko Maeda¹, Hideki Nagata, Masae Kuboniwa, Kosuke Kataoka, Nobuko Nishida, Muneo Tanaka, Satoshi Shizukuishi

Affiliation

¹ Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

Binding of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to Porphyromonas gingivalis fimbriae was characterized via a biomolecular interaction analysis system. The interaction was specific, and the association constant value was 4.34 x 10(7) M(-1), suggesting that S. oralis GAPDH functions as a dominant receptor for P. gingivalis and contributes to P. gingivalis colonization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques
Fimbriae Proteins / genetics
Fimbriae Proteins / metabolism
Fimbriae, Bacterial / metabolism*
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism*
Humans
Kinetics
Porphyromonas gingivalis / metabolism*
Streptococcus oralis / enzymology*
Streptococcus oralis / metabolism

Substances

fimbrillin
Fimbriae Proteins
Glyceraldehyde-3-Phosphate Dehydrogenases