Genetic and culture-based approaches for detecting macrolide resistance in Chlamydia pneumoniae

Antimicrob Agents Chemother. 2004 Sep;48(9):3586-90. doi: 10.1128/AAC.48.9.3586-3590.2004.

Abstract

Three clinical Chlamydia pneumoniae isolates for which the MIC of azithromycin increased after treatment were investigated for genetic evidence of macrolide resistance. Attempts to induce antibiotic resistance in vitro were made. No genetic mechanism was identified for the phenotypic change in these C. pneumoniae isolates. No macrolide resistance was obtained in vitro.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Azithromycin / pharmacology
  • Chlamydia Infections / microbiology
  • Chlamydophila pneumoniae / drug effects*
  • Chlamydophila pneumoniae / genetics*
  • Clarithromycin / pharmacology
  • DNA Primers
  • Drug Resistance, Bacterial
  • Humans
  • Macrolides / pharmacology*
  • Microbial Sensitivity Tests
  • Phenotype
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 23S / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Anti-Bacterial Agents
  • DNA Primers
  • Macrolides
  • RNA, Bacterial
  • RNA, Ribosomal, 23S
  • Azithromycin
  • Clarithromycin