A naturally occurring variation in the proline-rich region does not attenuate human immunodeficiency virus type 1 nef function

Elke Rücker; Jan Münch; Steffen Wildum; Matthias Brenner; Jutta Eisemann; Leonid Margolis; Frank Kirchhoff

doi:10.1128/JVI.78.18.10197-10201.2004

A naturally occurring variation in the proline-rich region does not attenuate human immunodeficiency virus type 1 nef function

J Virol. 2004 Sep;78(18):10197-201. doi: 10.1128/JVI.78.18.10197-10201.2004.

Authors

Elke Rücker¹, Jan Münch, Steffen Wildum, Matthias Brenner, Jutta Eisemann, Leonid Margolis, Frank Kirchhoff

Affiliation

¹ Department of Virology-Universitätsklinikum, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

Abstract

We analyzed human immunodeficiency virus type 1 (HIV-1) Nef variants to further evaluate the functional relevance of the R71T substitution previously proposed to attenuate viral replication (Fackler et al., Curr. Biol. 11:1294-1299, 2001). Our results demonstrate that this variation in the proline-rich region does not significantly affect the functional activity of Nef or HIV-1 infectivity or replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Gene Products, nef / chemistry
Gene Products, nef / genetics*
Gene Products, nef / physiology
Genes, nef
Genetic Variation
HIV-1 / genetics*
HIV-1 / pathogenicity
HIV-1 / physiology
HeLa Cells
Humans
Jurkat Cells
Mutagenesis, Site-Directed
Proline / chemistry
Virulence / genetics
Virus Replication / genetics
nef Gene Products, Human Immunodeficiency Virus

Substances

Gene Products, nef
nef Gene Products, Human Immunodeficiency Virus
Proline