Abstract
Expression of mRNAs encoding the erythroid-specific delta-aminolevulinate synthase (ALAS-E) and the nonspecific delta-aminolevulinate synthase (ALAS-N) were examined in murine Friend virus-transformed erythroleukemia (MEL) cells using nonradioactive in situ hybridization. Following dimethyl sulfoxide (DMSO) treatment, ALAS-E mRNA increased markedly, while ALAS-N mRNA did not increase in wild-type MEL cells. In contrast, in a DMSO-resistant clone of MEL cells, ALAS-E was not detectable before and after DMSO treatment. These findings suggest that ALAS-E and ALAS-N mRNAs are under separate controls and that the expression of ALAS-E mRNA is a critical event in erythroid differentiation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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5-Aminolevulinate Synthetase / genetics*
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Animals
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DNA, Neoplasm / genetics
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Dimethyl Sulfoxide / pharmacology
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Erythroid Precursor Cells / drug effects
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Erythroid Precursor Cells / enzymology
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Erythroid Precursor Cells / pathology
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Erythropoiesis / genetics*
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Erythropoiesis / physiology
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Friend murine leukemia virus
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / genetics*
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Gene Expression Regulation, Enzymologic / physiology
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Isoenzymes / genetics
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Leukemia, Erythroblastic, Acute / enzymology
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Leukemia, Erythroblastic, Acute / genetics
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Leukemia, Erythroblastic, Acute / pathology
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Mice
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Nucleic Acid Hybridization
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RNA, Messenger / drug effects
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RNA, Messenger / genetics*
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / enzymology
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Tumor Cells, Cultured / pathology
Substances
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DNA, Neoplasm
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Isoenzymes
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RNA, Messenger
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5-Aminolevulinate Synthetase
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Dimethyl Sulfoxide