A calcium-calmodulin antagonist blocks experimental Vibrio vulnificus cytolysin-induced lethality in an experimental mouse model

Infect Immun. 2004 Oct;72(10):6157-9. doi: 10.1128/IAI.72.10.6157-6159.2004.

Abstract

We demonstrated that trifluoperazine, a calcium-calmodulin antagonist, blocked the hyperpermeability induced by Vibrio vulnificus cytolysin in in vitro-modeled endothelium and prevented the deaths of mice. Furthermore, compared to tetracycline alone, tetracycline combined with trifluoperazine enhanced the survival rate of V. vulnificus-infected mice, indicating the role of the cytolysin as an important factor in pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / metabolism
  • Animals
  • Calcium / antagonists & inhibitors*
  • Calcium / metabolism
  • Calmodulin / antagonists & inhibitors*
  • Calmodulin / metabolism
  • Capillary Permeability / drug effects*
  • Cell Line
  • Cytotoxins / antagonists & inhibitors*
  • Cytotoxins / pharmacology
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Mice
  • Models, Animal*
  • Protein Transport / drug effects
  • Trifluoperazine / pharmacology*
  • Vibrio vulnificus / chemistry
  • Vibrio vulnificus / pathogenicity*
  • Vibrio vulnificus / physiology

Substances

  • Albumins
  • Calmodulin
  • Cytotoxins
  • Trifluoperazine
  • Calcium