Abstract
Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to the homologous rat sequences. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 2 were 90% and 98% identical, respectively, to the corresponding rat sequences. A human extracellular signal-regulated kinase 2 specific probe was used to demonstrate that the mRNA for this kinase was present in T cells and did not change with activation. The deduced protein sequences of both human kinases were greater than 95% identical to two Xenopus kinase sequences, indicating that these enzymes are highly conserved across species.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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DNA / genetics
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DNA / isolation & purification
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DNA, Neoplasm / genetics
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Humans
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Mice
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases*
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Molecular Sequence Data
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Monocytes / enzymology*
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Protein Kinases / genetics*
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Rats
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Sequence Homology, Nucleic Acid
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T-Lymphocytes / enzymology*
Substances
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DNA, Neoplasm
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RNA, Messenger
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DNA
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Protein Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
Associated data
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GENBANK/D10271
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GENBANK/D10272
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GENBANK/D10273
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GENBANK/D10274
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GENBANK/D10275
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GENBANK/D10276
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GENBANK/M84489
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GENBANK/M84490
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GENBANK/M84512
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GENBANK/M84513