Molecular cloning and sequencing of the gene encoding human angiotensin II type 1 receptor

Biochem Biophys Res Commun. 1992 Feb 28;183(1):8-13. doi: 10.1016/0006-291x(92)91600-u.

Abstract

The gene of human angiotensin II type 1 (AT1) receptor was isolated from a lymphocyte genomic library. The coding region of the human AT1 receptor gene was contained in a single exon coding segment of the gene indicating an intronless structure of the coding region. The amino acid sequence of human AT1 receptor deduced from its base sequence has 359 amino acids and showed a high degree of sequence identity to bovine and rat AT1 receptor sequences. Amino acid substitutions specific to the human AT1 receptor were mostly confined to the carboxy terminal half of the molecule. The seven-transmembrane domains are well-conserved in those sequences.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Gene Library
  • Humans
  • Lymphocytes / chemistry
  • Molecular Sequence Data
  • Protein Conformation
  • Receptors, Angiotensin / classification
  • Receptors, Angiotensin / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Receptors, Angiotensin

Associated data

  • GENBANK/Z11162