Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset

J Struct Biol. 2004 Sep;147(3):283-90. doi: 10.1016/j.jsb.2004.02.008.

Abstract

A method of supervised classification using two available structure templates was applied to investigate the possible heterogeneity existing in a large cryo-EM dataset of an Escherichia coli 70S ribosome-EF-G complex. Two subpopulations showing the ribosome in distinct conformational states, related by a ratchet-like rotation of the 30S subunit with respect to the 50S subunit, were extracted from the original dataset. The possible presence of additional intermediate states is discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cryoelectron Microscopy / methods
  • Escherichia coli / metabolism
  • Escherichia coli / ultrastructure
  • Fourier Analysis
  • Models, Molecular
  • Peptide Elongation Factor G / chemistry*
  • Peptide Elongation Factor G / metabolism*
  • Protein Conformation
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / metabolism
  • RNA, Transfer / chemistry
  • RNA, Transfer / metabolism
  • Ribosomes / metabolism*
  • Ribosomes / ultrastructure*

Substances

  • Peptide Elongation Factor G
  • RNA, Bacterial
  • RNA, Transfer