Abstract
We have used a novel phase-referenced heterodyne dual-beam low-coherence interferometer to perform what we believe are the first noncontact measurements of surface motion in a nerve bundle during the action potential. Nerve displacements of approximately 5-nm amplitude and approximately 10-ms duration are measured without signal averaging. This interferometer may find general application in measurement of small motion in cells and other weakly scattering samples.
Publication types
-
Comparative Study
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
-
Validation Study
MeSH terms
-
Action Potentials / physiology*
-
Animals
-
Microscopy, Interference / instrumentation*
-
Microscopy, Interference / methods*
-
Movement / physiology*
-
Nanotechnology / instrumentation
-
Nanotechnology / methods
-
Nephropidae
-
Nerve Fibers / physiology*
-
Nerve Fibers / ultrastructure*
-
Neural Conduction / physiology*
-
Peripheral Nerves / physiology
-
Peripheral Nerves / ultrastructure
-
Reproducibility of Results
-
Sensitivity and Specificity