IL-1 and TNF antagonists prevent inhibition of fracture healing by ethanol in rats

Toxicol Sci. 2004 Dec;82(2):656-60. doi: 10.1093/toxsci/kfi002. Epub 2004 Oct 6.

Abstract

We tested the hypothesis that combined administration of IL-1 and TNF antagonists would protect fracture healing from inhibition by chronic ethanol exposure. Adult male rats were fed a liquid diet +/- ethanol (CON and ETOH) by intragastric infusion for three weeks prior to and three weeks after creation of an externally fixated tibial fracture. Beginning the day of fracture, one-half of each dietary group received 2.0 mg/kg/day IL-1ra and 2.0 mg/kg/2-days sTNFR1 (CON + ANTAG and ETOH + ANTAG), while all other animals received vehicle alone (CON + VEH and ETOH + VEH). Scoring of ex vivo radiographs and analysis by pQCT revealed a significantly lower incidence of bridging and reduced total mineral content in the ETOH + VEH group compared to all other groups. These results support, for the first time, the hypothesis that IL-1 and TNF antagonists are capable of protecting fracture healing from the inhibition associated with chronic ethanol consumption.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bony Callus / drug effects
  • Central Nervous System Depressants / toxicity*
  • Ethanol / antagonists & inhibitors*
  • Ethanol / toxicity*
  • Fracture Healing / drug effects*
  • Interleukin-1 / antagonists & inhibitors*
  • Male
  • Osteogenesis / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Tumor Necrosis Factor, Type I / drug effects
  • Signal Transduction / drug effects
  • Tomography, X-Ray Computed
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors*
  • Weight Gain / drug effects

Substances

  • Central Nervous System Depressants
  • Interleukin-1
  • Receptors, Tumor Necrosis Factor, Type I
  • Tumor Necrosis Factor-alpha
  • Ethanol