Tubulin as a regulator of G-protein signaling

Methods Enzymol. 2004:390:389-403. doi: 10.1016/S0076-6879(04)90024-9.

Abstract

Tubulin is known to form high-affinity complexes with certain G proteins. The formation of such complexes allows tubulin to activate Galpha and fosters a system whereby elements of the cytoskeleton can influence G-protein signaling. This article describes the interaction between tubulin and G proteins and discusses methods for examining this interaction.

MeSH terms

  • Animals
  • Cell Line
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Cytoskeleton / metabolism
  • Enzyme Activation
  • GTP-Binding Protein alpha Subunits / genetics
  • GTP-Binding Protein alpha Subunits / metabolism
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • Glioma / metabolism
  • Guanosine Triphosphate / analogs & derivatives
  • Guanosine Triphosphate / metabolism
  • Isoenzymes / metabolism
  • Phospholipase C beta
  • Protein Binding
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Second Messenger Systems / physiology*
  • Tubulin / metabolism*
  • Type C Phospholipases / metabolism

Substances

  • GTP-Binding Protein alpha Subunits
  • Isoenzymes
  • Recombinant Fusion Proteins
  • Tubulin
  • Guanosine Triphosphate
  • Type C Phospholipases
  • Phospholipase C beta
  • GTP-Binding Proteins