Abstract
PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.
MeSH terms
-
Ascomycota / enzymology*
-
Ascomycota / genetics*
-
Bridged Bicyclo Compounds, Heterocyclic / metabolism*
-
DNA, Complementary / biosynthesis
-
DNA, Complementary / genetics
-
Gene Amplification
-
Peptide Library
-
Polyketide Synthases / genetics*
-
Promoter Regions, Genetic
-
Tricarboxylic Acids / metabolism*
Substances
-
Bridged Bicyclo Compounds, Heterocyclic
-
DNA, Complementary
-
Peptide Library
-
Tricarboxylic Acids
-
squalestatin 1
-
Polyketide Synthases