Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing

Keiji Itaka; Naoki Kanayama; Nobuhiro Nishiyama; Woo-Dong Jang; Yuichi Yamasaki; Kozo Nakamura; Hiroshi Kawaguchi; Kazunori Kataoka

doi:10.1021/ja047174r

Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing

J Am Chem Soc. 2004 Oct 27;126(42):13612-3. doi: 10.1021/ja047174r.

Authors

Keiji Itaka¹, Naoki Kanayama, Nobuhiro Nishiyama, Woo-Dong Jang, Yuichi Yamasaki, Kozo Nakamura, Hiroshi Kawaguchi, Kazunori Kataoka

Affiliation

¹ Department of Materials Science and Engineering, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

PMID: 15493907
DOI: 10.1021/ja047174r

Abstract

An siRNA nanocarrier formed through self-assembly of PEG-based block catiomer possessing two distinct amino groups with different pKa values in a side chain was developed. This design provided the carrier with a sufficient siRNA complexation and an assumed buffering capacity in the endosomes, allowing it to exhibit remarkable gene knockdown abilities as well as sufficient serum tolerability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cations / chemistry
DNA / chemistry
DNA / genetics
Diamines / chemistry*
Gene Silencing*
Humans
Kinetics
Nanostructures / chemistry
Peptides / chemistry
Polyethylene Glycols / chemistry*
RNA, Small Interfering / chemistry*
RNA, Small Interfering / genetics

Substances

Cations
Diamines
Peptides
RNA, Small Interfering
poly-beta-benzyl-aspartate
Polyethylene Glycols
DNA