Lipid rafts clustering and signalling by listeriolysin O

Biochem Soc Trans. 2004 Nov;32(Pt 5):712-4. doi: 10.1042/BST0320712.

Abstract

Listeriolysin O, the major virulent determinant of Listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. In the present study, we reveal its other facet, rafts clustering. By immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins CD14 and CD24, which normally exhibit uniform distribution on J774 cells, undergo clustering upon treatment with LLO. The non-raft marker transferrin receptor is unaffected by such treatment. Rafts clustering might explain the induction of tyrosine phosphorylation observed on LLO-treated cells.

Publication types

  • Review

MeSH terms

  • Antigens, CD / biosynthesis
  • Bacterial Toxins / pharmacology*
  • CD24 Antigen
  • Cell Line
  • Cell Membrane / metabolism
  • Cholesterol / chemistry
  • Heat-Shock Proteins / pharmacology*
  • Hemolysin Proteins
  • Humans
  • Lipopolysaccharide Receptors / biosynthesis
  • Listeria monocytogenes / metabolism
  • Membrane Glycoproteins / biosynthesis
  • Membrane Microdomains / chemistry*
  • Microscopy, Fluorescence
  • Models, Biological
  • Phosphorylation
  • Signal Transduction
  • Tyrosine / chemistry

Substances

  • Antigens, CD
  • Bacterial Toxins
  • CD24 Antigen
  • CD24 protein, human
  • Heat-Shock Proteins
  • Hemolysin Proteins
  • Lipopolysaccharide Receptors
  • Membrane Glycoproteins
  • Tyrosine
  • Cholesterol
  • hlyA protein, Listeria monocytogenes