Gene expression analysis of peripheral T cells in a subgroup of common variable immunodeficiency shows predominance of CCR7(-) effector-memory T cells

Clin Exp Immunol. 2004 Nov;138(2):278-89. doi: 10.1111/j.1365-2249.2004.02630.x.

Abstract

Common variable immunodeficiency (CVID) represents a heterogeneous group of antibody deficiency syndromes, characterized by defective antibody production in which T cell deficiency may play a pathogenic role. A subgroup of CVID patients has impaired in vitro T cell proliferation. Using microarray analyses of T cells from these patients, we found a gene expression pattern different from healthy controls and patients with X-linked agammaglobulinaemia. The profile of the differentially expressed genes suggests enhanced cytotoxic effector functions, antigen experienced or chronically activated T cells and a predominance of CCR7(-) T cells. Further experiments using flow cytometry revealed a striking predominance of CCR7(-) T cells in a subgroup of CVID patients, and an association with impaired T cell proliferation. Our observations indicate that a predominance of CCR7(-) T cells with effector-memory cell features and with reduced proliferative capacity may characterize a subgroup of CVID.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Agammaglobulinemia / genetics
  • Agammaglobulinemia / immunology
  • Aged
  • Cell Division / genetics
  • Cell Division / immunology
  • Cells, Cultured
  • Chromosomes, Human, X
  • Common Variable Immunodeficiency / genetics
  • Common Variable Immunodeficiency / immunology*
  • Female
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / immunology*
  • Genetic Linkage / genetics
  • Genetic Linkage / immunology
  • Humans
  • Immunologic Memory / immunology
  • Lymphocyte Activation / genetics
  • Lymphocyte Activation / immunology
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis / methods
  • Receptors, CCR7
  • Receptors, Chemokine / immunology*
  • T-Lymphocytes / immunology*
  • Transcription, Genetic

Substances

  • CCR7 protein, human
  • Receptors, CCR7
  • Receptors, Chemokine