Abstract
Clinical isolates of Streptococcus pyogenes were classified by serological typing of their surface M protein. Non-M typeable strains with the emm1 gene were characterized as the degradation of M protein caused by overproduction of the extracellular cysteine protease, SpeB. These events are dependent on the growth phase. M protein produced prior to expression of SpeB is degraded in the stationary phase when the active form of SpeB is detected. The proteolytic degradation of M protein should be considered for precise M typing analysis.
MeSH terms
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Antigens, Bacterial / genetics
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Antigens, Bacterial / isolation & purification*
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / isolation & purification*
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Bacterial Proteins
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification*
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Cysteine Endopeptidases / analysis
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism*
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Cysteine Endopeptidases / physiology
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Humans
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Serotyping
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Streptococcus pyogenes / enzymology*
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Streptococcus pyogenes / genetics
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Streptococcus pyogenes / immunology
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Streptococcus pyogenes / isolation & purification
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Virulence
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Carrier Proteins
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streptococcal M protein
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Cysteine Endopeptidases
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streptopain