Matrix metalloproteinases and their inhibitors in the cervical mucus plug at term of pregnancy

Am J Obstet Gynecol. 2004 Oct;191(4):1232-9. doi: 10.1016/j.ajog.2004.03.023.

Abstract

Objective: This study was undertaken to evaluate the presence of matrix metalloproteinases (MMPs) and their inhibitors in the cervical mucus plug obtained during active labor at term.

Study design: Cervical mucus plugs from 17 healthy women in normal active labor were homogenized, extracted, and analyzed by 4 different assays.

Results: Gelatin zymography revealed large amounts of MMP-2 and MMP-9, and triple-helical collagen degradation demonstrated collagenase activity (MMP-8). Reverse zymography showed that tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and TIMP-2 are detectable in the cervical mucus plug. Quantification by enzyme-linked immunosorbent assay confirmed the presence of MMP-2, MMP-8, MMP-9, and TIMP-1.

Conclusion: Our results suggest that proteolytic or nonproteolytic effects of MMP and TIMP may play a part in the function of the cervical mucus plug of pregnancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cervix Mucus / chemistry*
  • Cervix Mucus / diagnostic imaging
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Labor, Obstetric / physiology*
  • Matrix Metalloproteinase 2 / analysis
  • Matrix Metalloproteinase 8 / analysis
  • Matrix Metalloproteinase 9 / analysis
  • Matrix Metalloproteinases / analysis*
  • Pregnancy
  • Tissue Inhibitor of Metalloproteinases / analysis*
  • Ultrasonography, Prenatal

Substances

  • Tissue Inhibitor of Metalloproteinases
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 8
  • Matrix Metalloproteinase 9