Synthesis of long Poly(dG).Poly(dC) DNA using enzymatic reaction

Shin-ichi Tanaka; Masateru Taniguchi; Susumu Uchiyama; Kiichi Fukui; Tomoji Kawai

doi:10.1039/b410753e

Synthesis of long Poly(dG).Poly(dC) DNA using enzymatic reaction

Chem Commun (Camb). 2004 Nov 7:(21):2388-9. doi: 10.1039/b410753e. Epub 2004 Sep 21.

Authors

Shin-ichi Tanaka¹, Masateru Taniguchi, Susumu Uchiyama, Kiichi Fukui, Tomoji Kawai

Affiliation

¹ Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0074, Japan.

PMID: 15514779
DOI: 10.1039/b410753e

Abstract

Non-defect Poly(dG).Poly(dC) of 500 bp (170 nm) has been synthesized by using enzymatic reactions and was characterized by its UV spectrum, showing that conjugated pi-electrons between base pairs are spread over the DNA molecule suggesting the absence of structural defects.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemical synthesis*
DNA Ligases / chemistry*
Electrons
Polydeoxyribonucleotides / chemical synthesis*
Spectrophotometry, Ultraviolet

Substances

Polydeoxyribonucleotides
poly(dG).poly(dC)
DNA
DNA Ligases