Sequence analysis of pDN571, a plasmid encoding novel bacteriocin production in M-type 57 Streptococcus pyogenes

Nicholas C K Heng; Grace A Burtenshaw; Ralph W Jack; John R Tagg

doi:10.1016/j.plasmid.2004.08.002

Sequence analysis of pDN571, a plasmid encoding novel bacteriocin production in M-type 57 Streptococcus pyogenes

Plasmid. 2004 Nov;52(3):225-9. doi: 10.1016/j.plasmid.2004.08.002.

Authors

Nicholas C K Heng¹, Grace A Burtenshaw, Ralph W Jack, John R Tagg

Affiliation

¹ Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. [email protected]

PMID: 15518879
DOI: 10.1016/j.plasmid.2004.08.002

Abstract

Production of the novel bacteriocin streptococcin A-M57 (SA-M57) by Streptococcus pyogenes strains of M-protein type 57 is plasmid-associated. Plasmid pDN571 (3351bp) harbored by S. pyogenes 71-724, the prototype M-type 57 strain, has been completely sequenced and contains three putative open reading frames (repA, scnM57 and ORF3). In addition, the double-strand and single-strand (SSO) origins of replication were identified. Analysis of the replication-associated genetic elements places pDN571 in the ubiquitous pC194/pUB110 family of rolling-circle plasmids. The SSO of pDN571 is of the ssoA type. SA-M57 (encoded by scnM57) is synthesized as a secreted 179-amino acid polypeptide with a 27-residue secretion signal peptide and has no homology to proteins of known function.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteriocins / genetics*
Molecular Sequence Data
Open Reading Frames
Plasmids / chemistry*
Plasmids / genetics
Restriction Mapping
Streptococcus pyogenes / genetics*

Substances

Bacteriocins