Molecular detection of linezolid resistance in Enterococcus faecium and Enterococcus faecalis by use of 5' nuclease real-time PCR compared to a modified classical approach

J Clin Microbiol. 2004 Nov;42(11):5327-31. doi: 10.1128/JCM.42.11.5327-5331.2004.

Abstract

A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.

Publication types

  • Evaluation Study

MeSH terms

  • Acetamides / pharmacology*
  • Alleles
  • Anti-Infective Agents / pharmacology*
  • DNA, Bacterial / analysis
  • Drug Resistance, Bacterial / genetics*
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecium / drug effects*
  • Humans
  • Linezolid
  • Oligonucleotide Array Sequence Analysis
  • Oxazolidinones / pharmacology*
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 23S / genetics
  • Taq Polymerase

Substances

  • Acetamides
  • Anti-Infective Agents
  • DNA, Bacterial
  • Oxazolidinones
  • RNA, Ribosomal, 23S
  • Taq Polymerase
  • Linezolid