Abstract
General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
MeSH terms
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Anti-Infective Agents, Local / chemistry*
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DNA, Bacterial / analysis*
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DNA, Bacterial / genetics
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Endophthalmitis / diagnosis*
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Endophthalmitis / microbiology
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Equipment Contamination*
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Eye Infections, Bacterial / diagnosis*
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Eye Infections, Bacterial / microbiology
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Humans
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Povidone-Iodine / chemistry*
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Pseudomonas / chemistry
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Pseudomonas / genetics
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Vitrectomy
Substances
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Anti-Infective Agents, Local
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DNA, Bacterial
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Povidone-Iodine