A comparative study of clinically well-characterized human atherosclerotic plaques with histological, chemical, and ultrastructural methods

J Inorg Biochem. 2004 Dec;98(12):2032-8. doi: 10.1016/j.jinorgbio.2004.09.006.

Abstract

Atherosclerotic plaques (six cases) with well-documented clinical history were analysed using histology, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray (EDX) spectroscopy, infrared spectroscopy (IR), thermogravimetry (TG), and high-resolution synchrotron X-ray diffraction. All samples contained about 60-70 wt% biological carbonated apatite (in dry state) in a nanocrystalline form with particle sizes of about 20 nm. Structurally, there are strong similarities to bone mineral. Ultrastructural investigations documented typical calcospherites, mineralisation processes starting at collagen fibrils and ring-shaped crystalline mineralised structures. There were no significant ultrastructural or chemical differences between the calcifications of individual patients.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Aorta / pathology*
  • Aorta / ultrastructure*
  • Arteriosclerosis / diagnosis
  • Arteriosclerosis / pathology*
  • Calcinosis / pathology*
  • Female
  • Humans
  • Male
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Middle Aged
  • Spectrophotometry, Infrared
  • Synchrotrons
  • Thermogravimetry
  • X-Ray Diffraction