Establishment of T cell-specific and natural killer cell-specific unigene sets: towards high-throughput genomics of leukaemia

J Illiger; R Herwig; M Steinfath; T Przewieslik; T Elge; Ch Bull; U Radelof; H Lehrach; M Janitz

doi:10.1111/j.1365-2370.2004.00483.x

Establishment of T cell-specific and natural killer cell-specific unigene sets: towards high-throughput genomics of leukaemia

Eur J Immunogenet. 2004 Dec;31(6):253-7. doi: 10.1111/j.1365-2370.2004.00483.x.

Authors

J Illiger¹, R Herwig, M Steinfath, T Przewieslik, T Elge, Ch Bull, U Radelof, H Lehrach, M Janitz

Affiliation

¹ Max Planck Institute for Molecular Genetics, Berlin, Germany.

PMID: 15548262
DOI: 10.1111/j.1365-2370.2004.00483.x

Abstract

We report the establishment of highly non-redundant unigene sets consisting of cDNA clones derived from T lymphocytes and natural killer cells. Each set consists of 10 506 and 13 409 clones, respectively, arrayed on nylon membranes in duplicate. The sets provide an excellent tool for genome-wide gene expression analysis studies in immunology research.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Northern
DNA, Complementary / metabolism
Gene Expression / physiology*
Gene Expression Profiling
Gene Library
Humans
Jurkat Cells
Killer Cells, Natural / metabolism*
Leukemia / genetics*
Leukemia / metabolism*
Male
T-Lymphocytes / metabolism*

Substances

DNA, Complementary