Abstract
Objective:
To elucidate the expression of tanscription factor Ets-1 mediated by EB virus encoded latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells.
Methods:
LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2) were used. Expression of LMP1 and Ets-1 was observed after induction with Doxycycline (Dox). Expression of Ets-1 mRNA and protein was detected by RT-PCR and Western blot, respectively. The phosphorylation level of Ets-1 protein was examined by co-immunoprecipitation. The DNA binding activity of Ets-1 was detected by electrophoretic-mobility shift assay (EMSA).
Results:
After induction with Dox in pTet-on-LMP1 HNE2 cells, to some extent, the expression of Ets-1 mRNA and protein, its phosphorylation level and DNA binding activity were increased with enhancement of LMP1 expression.
Conclusion:
LMP1 induces transcriptional activation and expression of Ets-1 which may contribute to the development of NPC.
Publication types
-
English Abstract
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Carcinoma, Squamous Cell / metabolism*
-
Carcinoma, Squamous Cell / pathology
-
Carcinoma, Squamous Cell / virology
-
Cell Line, Tumor
-
Doxycycline / pharmacology
-
Herpesvirus 4, Human*
-
Humans
-
Nasopharyngeal Neoplasms / metabolism*
-
Nasopharyngeal Neoplasms / pathology
-
Nasopharyngeal Neoplasms / virology
-
Phosphorylation
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins / biosynthesis*
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins c-ets
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Transcription Factors / biosynthesis*
-
Transcription Factors / genetics
-
Viral Matrix Proteins / biosynthesis*
-
Viral Matrix Proteins / genetics
-
Viral Matrix Proteins / physiology
Substances
-
EBV-associated membrane antigen, Epstein-Barr virus
-
ETS1 protein, human
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-ets
-
RNA, Messenger
-
Transcription Factors
-
Viral Matrix Proteins
-
Doxycycline