Axis curvature and ligand induced bending in the CAP-DNA oligomers

Biophys J. 2005 Jan;88(1):L04-6. doi: 10.1529/biophysj.104.053058. Epub 2004 Nov 19.

Abstract

The origin of DNA axis curvature in complexes of the catabolite activator protein with DNA is studied using multiple molecular dynamics (MD) simulations of the free and protein-bound forms of the DNA. The results are compared to available solution and crystal structure data. The MD simulations reproduce the experimentally observed bend in DNA and indicate that ∼40% of the bending observed in the complex is intrinsic to the DNA sequence, whereas ∼60% is induced on protein binding. The MD provides a model for the dynamical structure of the DNA free in solution and for ligand-induced bending.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biophysics / methods
  • Computational Biology / methods*
  • Computer Simulation
  • Crystallography, X-Ray / methods
  • Cyclic AMP Receptor Protein / chemistry*
  • DNA / chemistry*
  • Escherichia coli / metabolism
  • Ligands
  • Nucleic Acid Conformation*
  • Protein Binding
  • Protein Conformation
  • Proteins / chemistry
  • Software
  • Thermodynamics
  • Time Factors

Substances

  • Cyclic AMP Receptor Protein
  • Ligands
  • Proteins
  • DNA