Abstract
The distribution and organization of nucleolar DNA in Vicia faba L. was analyzed by specific cytochemical staining using NAMA-Ur. The results showed that nucleolar DNA was distributed in the FCs and at the FC/DFC junctions. Statistical analysis showed that the rRNA genes occupied about one-third of the total dense fibrillar component region. The rDNA was condensed in some regions and uncondensed in others. Nucleolus-associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, suggesting a possible origin for nucleolar DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Nucleolus / genetics*
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Cell Nucleolus / ultrastructure*
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Chromatin / genetics
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Chromatin / ultrastructure
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Coloring Agents
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics*
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Histocytochemistry / methods
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Microscopy, Electron, Transmission
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Organometallic Compounds
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Plant Roots / genetics
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Plant Roots / ultrastructure
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Staining and Labeling / methods*
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Transcription, Genetic / genetics
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Vicia faba / genetics*
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Vicia faba / ultrastructure
Substances
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Chromatin
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Coloring Agents
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DNA, Ribosomal
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Organometallic Compounds
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uranyl acetate