NF-kappaB precursor, p105, and NF-kappaB inhibitor, IkappaBgamma, are both elevated in Alzheimer disease brain

Neurosci Lett. 2005 Jan 10;373(2):115-8. doi: 10.1016/j.neulet.2004.09.074.

Abstract

The nuclear factor-kappaB (NF-kappaB) signal transduction pathway regulates several vital cellular processes. During our studies of the glycoproteins involved in Alzheimer disease (AD), we found a significant increase of a 45-kDa protein band that was stained by lectin Maackia amurensis agglutinin (MAA). Mass spectrometry and Western blot analyses indicated that this 45-kDa MMA-positive protein was an inhibitor of NF-kappaB, IkappaBgamma. By Western blot analysis, the levels of both IkappaBgamma and NF-kappaB precursor, p105, were found to be elevated in AD brain as compared to age-matched controls. Our findings suggest that the NF-kappaB pathway might be involved in the molecular mechanism of AD.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aged
  • Alzheimer Disease / metabolism*
  • Amino Acid Sequence
  • Antigens, Nuclear / metabolism*
  • Blotting, Western
  • Cerebral Cortex / metabolism*
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • I-kappa B Proteins / genetics
  • I-kappa B Proteins / metabolism*
  • Mass Spectrometry
  • NF-kappa B / metabolism*
  • NF-kappa B p50 Subunit

Substances

  • Antigens, Nuclear
  • Chromosomal Proteins, Non-Histone
  • I-kappa B Proteins
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • NFKB1 protein, human