Abstract
Myeloid, CD1a-sorted dendritic cells (MDC) productively replicated human immunodeficiency virus strains encoding envelope genes of either primary X4R5 or R5 strains for up to 45 days. Cell-free supernatant collected from long-term infected MDC, which had been exposed to an X4R5 virus 45 days earlier, was still infectious when placed over activated T cells. These data imply that DC can act as a persistent reservoir of infectious virus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD1 / metabolism*
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Cell Differentiation
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Cells, Cultured
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Dendritic Cells / virology*
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Disease Reservoirs
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HIV Infections / virology*
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HIV-1 / pathogenicity*
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HIV-1 / physiology
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Humans
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Myeloid Cells / cytology*
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Receptors, CCR5 / metabolism
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Receptors, CXCR4 / metabolism
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Time Factors
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Virus Replication
Substances
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Antigens, CD1
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CD1a antigen
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Receptors, CCR5
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Receptors, CXCR4