Flow cytometric analysis of human antigen-specific T-cell proliferation

Methods Cell Biol. 2004:75:481-96. doi: 10.1016/s0091-679x(04)75019-0.

Authors

Jason M Brenchley¹, Daniel C Douek

Affiliation

¹ Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20874, USA.

PMID: 15603438
DOI: 10.1016/s0091-679x(04)75019-0

No abstract available

MeSH terms

CD4-Positive T-Lymphocytes / cytology
CD4-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / cytology
CD8-Positive T-Lymphocytes / immunology*
Cell Proliferation*
Cellular Senescence / immunology
Flow Cytometry / methods*
Fluoresceins / chemistry
HIV Infections / immunology
Humans
Immunologic Factors / pharmacology
Immunophenotyping / trends
Leukocytes, Mononuclear / drug effects
Leukocytes, Mononuclear / immunology
Lymphocyte Activation / immunology
Monitoring, Immunologic / methods
Monitoring, Immunologic / trends
Signal Processing, Computer-Assisted
Staining and Labeling / methods
Succinimides / chemistry
Transplantation, Homologous / immunology
Vaccines / immunology

Substances

5-(6)-carboxyfluorescein diacetate succinimidyl ester
Fluoresceins
Immunologic Factors
Succinimides
Vaccines