Abstract
WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38, a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed during exposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38 genomic DNA was sequenced and mapped onto the centromeric region of the barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cell Nucleus / metabolism
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Cold Temperature
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DNA, Plant / chemistry
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DNA, Plant / genetics
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Electrophoretic Mobility Shift Assay
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Exons
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Gene Expression Regulation, Plant / drug effects
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Genes, Plant / genetics
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Hordeum / drug effects
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Hordeum / genetics*
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Introns
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Molecular Sequence Data
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Oligonucleotides / genetics
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Oligonucleotides / metabolism
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Water / pharmacology
Substances
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DNA, Plant
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Oligonucleotides
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Plant Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Transcription Factors
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Water
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Green Fluorescent Proteins