Abstract
The arachidonate cascade is important for the generation of reactive species (RS), and cyclooxygenase (COX) is a key enzyme of this cascade. Tissues of 24-month-old rat lung showed a 2-fold increase in RS, malondialdehyde and thromboxane B2 than those of 6-month-old rat. We found that the effects of 50 microM H2O2 and 200 microM t-butylhydroperoxide (t-BHP) specify on COX activity, and that their effects increased cytosolic COX activity in a concentration-dependent manner (1-50 microM) in 24-month-old rat. Our results suggested that COX activators such as t-BHP and H2O2, which are located in cytosol, are essential for the activation of COX in aged lung.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging / drug effects
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Aging / metabolism*
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Animals
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Dose-Response Relationship, Drug
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Enzyme Activation / drug effects
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Hydrogen Peroxide / pharmacology*
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Lung / drug effects*
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Lung / enzymology*
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Male
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Oxidative Stress / drug effects
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Oxidative Stress / physiology
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Prostaglandin-Endoperoxide Synthases / drug effects
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Prostaglandin-Endoperoxide Synthases / metabolism*
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Rats
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Rats, Inbred F344
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Reactive Oxygen Species / agonists
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Reactive Oxygen Species / metabolism*
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tert-Butylhydroperoxide / pharmacology*
Substances
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Reactive Oxygen Species
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tert-Butylhydroperoxide
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Hydrogen Peroxide
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Prostaglandin-Endoperoxide Synthases