Abstract
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Flow Cytometry / methods*
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Gene Expression Profiling / methods
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Gene Expression Regulation, Plant / physiology
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Hardness
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Hardness Tests / methods*
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Plant Proteins / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Seeds / physiology*
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Statistics as Topic
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Triticum / physiology*
Substances
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Plant Proteins
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puroindoline protein, Triticum aestivum