Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing

Marjo Haanperä; Pentti Huovinen; Jari Jalava

doi:10.1128/AAC.49.1.457-460.2005

Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing

Antimicrob Agents Chemother. 2005 Jan;49(1):457-60. doi: 10.1128/AAC.49.1.457-460.2005.

Authors

Marjo Haanperä¹, Pentti Huovinen, Jari Jalava

Affiliation

¹ Human Microbial Ecology Laboratory, National Public Health Institute, Kiinamyllynkatu 13, FI-20520 Turku, Finland. [email protected]

Abstract

A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacteria / drug effects
Base Sequence
DNA, Ribosomal / analysis
DNA, Ribosomal / genetics
Diphosphates / metabolism*
Drug Resistance, Bacterial / genetics*
Genes, rRNA
Humans
Macrolides / pharmacology*
Microbial Sensitivity Tests / methods
Molecular Sequence Data
Mutation*
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 23S / genetics*
Sequence Analysis, DNA

Substances

Anti-Bacterial Agents
DNA, Ribosomal
Diphosphates
Macrolides
RNA, Ribosomal, 23S