A method for calling gains and losses in array CGH data

Biostatistics. 2005 Jan;6(1):45-58. doi: 10.1093/biostatistics/kxh017.

Abstract

Array CGH is a powerful technique for genomic studies of cancer. It enables one to carry out genome-wide screening for regions of genetic alterations, such as chromosome gains and losses, or localized amplifications and deletions. In this paper, we propose a new algorithm 'Cluster along chromosomes' (CLAC) for the analysis of array CGH data. CLAC builds hierarchical clustering-style trees along each chromosome arm (or chromosome), and then selects the 'interesting' clusters by controlling the False Discovery Rate (FDR) at a certain level. In addition, it provides a consensus summary across a set of arrays, as well as an estimate of the corresponding FDR. We illustrate the method using an application of CLAC on a lung cancer microarray CGH data set as well as a BAC array CGH data set of aneuploid cell strains.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Chromosome Aberrations*
  • Cluster Analysis
  • Gene Dosage
  • Genome, Human
  • Humans
  • Lung Neoplasms / genetics
  • Nucleic Acid Hybridization / methods*
  • Oligonucleotide Array Sequence Analysis / methods*