Abstract
The MAP kinase pathway inhibitor U0126 caused phosphorylation and activation of AMP-activated protein kinase (AMPK) and increased phosphorylation of its downstream target acetyl-CoA carboxylase, in HEK293 cells. This effect only occurred in cells expressing the upstream kinase, LKB1. Of two other widely used MAP kinase pathway inhibitors not closely related in structure to U0126, PD98059 also activated AMPK but PD184352 did not. U0126 and PD98059, but not PD184352, also increased the cellular ADP:ATP and AMP:ATP ratios, accounting for their ability to activate AMPK. These results suggest the need for caution in interpreting experiments conducted using U0126 and PD98059.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AMP-Activated Protein Kinases
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Adenosine Monophosphate / metabolism*
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Adenosine Triphosphate / metabolism*
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Butadienes / pharmacology*
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Enzyme Inhibitors / pharmacology*
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Flavonoids / pharmacology*
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HeLa Cells
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Humans
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MAP Kinase Signaling System / drug effects*
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Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 1 / physiology
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Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 3 / physiology
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinases / physiology
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Multienzyme Complexes / metabolism*
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Nitriles / pharmacology*
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Phosphorylation / drug effects
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Protein Serine-Threonine Kinases / metabolism*
Substances
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Butadienes
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Enzyme Inhibitors
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Flavonoids
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Multienzyme Complexes
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Nitriles
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U 0126
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Adenosine Monophosphate
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Adenosine Triphosphate
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Protein Serine-Threonine Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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AMP-Activated Protein Kinases
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one