Synthesis, characterization, DNA-binding and photocleavage studies of [Ru(bpy)2(PPIP)]2+ and [Ru(phen)2(PPIP)]2+

Li-Feng Tan; Hui Chao; Hong Li; Yun-Jun Liu; Bin Sun; Wei Wei; Liang-Nian Ji

doi:10.1016/j.jinorgbio.2004.10.028

Synthesis, characterization, DNA-binding and photocleavage studies of [Ru(bpy)2(PPIP)]2+ and [Ru(phen)2(PPIP)]2+

J Inorg Biochem. 2005 Feb;99(2):513-20. doi: 10.1016/j.jinorgbio.2004.10.028.

Authors

Li-Feng Tan¹, Hui Chao, Hong Li, Yun-Jun Liu, Bin Sun, Wei Wei, Liang-Nian Ji

Affiliation

¹ Department of Chemistry, Sun Yat-Sen University, Guangzhou 510275, PR China.

PMID: 15621284
DOI: 10.1016/j.jinorgbio.2004.10.028

Abstract

A novel ligand 2-(4'-phenoxy-phenyl)imidazo[4,5-f][1,10]phenanthroline (PPIP) and its complexes [Ru(bpy)(2)(PPIP)](2+) (1) (bpy = 2,2'-bipyridine) and [Ru(phen)(2)(PPIP)](2+) (2) (phen = 1,10-phenanthroline) have been synthesized and characterized by mass spectroscopy, (1)H NMR and cyclic voltammetry. The interaction of two complexes with calf thymus DNA (CT-DNA) was investigated by spectroscopic and viscosity measurements. The results suggest that both complexes bind to DNA via an intercalative mode. Both complexes have also been found to promote the photocleavage of plasmid pBR 322 DNA under irradiated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
DNA / drug effects
In Vitro Techniques
Intercalating Agents / chemical synthesis
Intercalating Agents / chemistry
Intercalating Agents / pharmacology
Magnetic Resonance Spectroscopy
Molecular Structure
Organometallic Compounds / chemical synthesis*
Organometallic Compounds / chemistry*
Organometallic Compounds / pharmacology
Phenanthrolines / chemical synthesis*
Phenanthrolines / chemistry*
Phenanthrolines / pharmacology
Photochemistry
Plasmids / drug effects
Ruthenium
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry

Substances

Intercalating Agents
Organometallic Compounds
Phenanthrolines
Ruthenium
DNA