Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1

Annabel Rector; Ruth Tachezy; Koenraad Van Doorslaer; Tracey MacNamara; Robert D Burk; John P Sundberg; Marc Van Ranst

doi:10.1016/j.virol.2004.10.033

Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1

Virology. 2005 Jan 20;331(2):449-56. doi: 10.1016/j.virol.2004.10.033.

Authors

Annabel Rector¹, Ruth Tachezy, Koenraad Van Doorslaer, Tracey MacNamara, Robert D Burk, John P Sundberg, Marc Van Ranst

Affiliation

¹ Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium.

PMID: 15629787
DOI: 10.1016/j.virol.2004.10.033

Abstract

The complete genome of a novel papillomavirus was isolated from a cutaneous papillomatous lesion of a North American porcupine (Erethizon dorsatum) using multiply primed rolling-circle amplification (RCA). The nucleotide sequence, genome organization, and phylogenetic position of the Erethizon dorsatum papillomavirus type 1 (EdPV-1) were determined. EdPV-1 is only distantly related to other benign cutaneous papillomavirus sequences and is the first member of the novel Sigma papillomavirus genus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cloning, Molecular
DNA, Circular / analysis*
DNA, Viral / analysis*
Dermatomycoses / microbiology
Dermatomycoses / veterinary*
Genome, Viral
North America
Nucleic Acid Amplification Techniques
Papillomaviridae / classification
Papillomaviridae / genetics*
Papillomaviridae / isolation & purification
Porcupines / virology*

Substances

DNA, Circular
DNA, Viral