Comparison of the Mycoplasma Duo test with PCR for detection of ureaplasma species in endotracheal aspirates from premature infants

Fook-Choe Cheah; Trevor P Anderson; Brian A Darlow; David R Murdoch

doi:10.1128/JCM.43.1.509-510.2005

Comparison of the Mycoplasma Duo test with PCR for detection of ureaplasma species in endotracheal aspirates from premature infants

J Clin Microbiol. 2005 Jan;43(1):509-10. doi: 10.1128/JCM.43.1.509-510.2005.

Authors

Fook-Choe Cheah¹, Trevor P Anderson, Brian A Darlow, David R Murdoch

Affiliation

¹ Department of Pathology, Christchurch School of Medicine and Health Sciences, University of Otago, P.O. Box 4345, Christchurch, New Zealand.

Abstract

We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma infection.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Culture Media
Humans
Infant, Newborn
Infant, Premature
Infant, Premature, Diseases / microbiology*
Polymerase Chain Reaction
Reagent Kits, Diagnostic*
Suction
Trachea / microbiology*
Ureaplasma / genetics
Ureaplasma / isolation & purification*
Ureaplasma Infections / microbiology*

Substances

Culture Media
Reagent Kits, Diagnostic