IgE- and IgE+Ag-mediated mast cell migration in an autocrine/paracrine fashion

Blood. 2005 Apr 15;105(8):3222-9. doi: 10.1182/blood-2004-11-4205. Epub 2005 Jan 6.

Abstract

Mast cells are the major effector cells for immediate hypersensitivity and chronic allergic reactions. These cells accumulate in mucosal tissues of allergic reactions, where immunoglobulin E (IgE) is produced locally. Here we provide evidence that, in addition to antigen that can attract IgE-bound mast cells, the type of IgE molecules that efficiently activate mast cells can promote the migration of mast cells in the absence of antigen. IgE- and IgE+Ag-mediated migration involves an autocrine/paracrine secretion of soluble factors including adenosine, leukotriene B4, and several chemokines. Their secretion depends on 2 tyrosine kinases, Lyn and Syk, and they are agonists of G-protein-coupled receptors and signal through phosphatidylinositol 3-kinase gamma, leading to mast cell migration. In mouse experiments, naive mast cells are attracted to IgE, and IgE-sensitized mast cells are attracted to antigen. Therefore, IgE and antigen are implicated in mast cell accumulation at allergic tissue sites with local high IgE levels.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens / immunology
  • Antigens / pharmacology
  • Autocrine Communication / immunology
  • Cell Movement / immunology*
  • Chemokines / pharmacology
  • Immunoglobulin E / immunology*
  • Immunoglobulin E / pharmacology
  • Integrins / metabolism
  • Mast Cells / cytology*
  • Mast Cells / immunology*
  • Mast Cells / metabolism
  • Mice
  • Mice, Mutant Strains
  • Paracrine Communication / immunology
  • Phosphatidylinositol 3-Kinases / metabolism
  • Receptors, G-Protein-Coupled / metabolism
  • Receptors, IgE / metabolism

Substances

  • Antigens
  • Chemokines
  • Integrins
  • Receptors, G-Protein-Coupled
  • Receptors, IgE
  • Immunoglobulin E
  • Phosphatidylinositol 3-Kinases