Genomic DNAs of 37 cultivars of yellow peach (Amgdalus persica) were amplified by RAPD (Randomly Amplified Polymorphic DNA) with 22 primers selected from 200 arbitrary 10 decamer primers. Phylogenetic relationship was analyzed through cluster of bands in 180 amplified sites. Molecular checking index and conservation of key germplasm were put forward according to special bands.