Expression and characterization of human tumor necrosis factor-related apoptosis inducing ligand in methylotrophic yeast Pichia pastoris

Jinzhi Wang; Juxing Chen; Mian Wu

Expression and characterization of human tumor necrosis factor-related apoptosis inducing ligand in methylotrophic yeast Pichia pastoris

Ital J Biochem. 2004 Jul;53(2):67-72.

Authors

Jinzhi Wang¹, Juxing Chen, Mian Wu

Affiliation

¹ Department of Molecular and Cellular Biology and Key Laboratory of Structural Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, PR China.

PMID: 15646010

Abstract

Recombinant human TRAIL was successfully expressed in a secreted form in methyltrophic yeast Pichia pastoris induced by methanol. The expressive product was immunoreactive to TRAIL antibody. The addition of the expressive product into cultured human lung cancer cell A549 showed moderate cell death, typical morphological characterization of apoptotic bodies, and DNA fragmentation. This result provided a new method to produce recombinant human TRAIL as a cancer therapeutic drug.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
Apoptosis Regulatory Proteins
Cell Line, Tumor / drug effects
Electroporation
Humans
Lung Neoplasms / metabolism
Membrane Glycoproteins / biosynthesis*
Membrane Glycoproteins / metabolism
Pichia / metabolism*
TNF-Related Apoptosis-Inducing Ligand
Tumor Necrosis Factor-alpha / biosynthesis*
Tumor Necrosis Factor-alpha / metabolism

Substances

Apoptosis Regulatory Proteins
Membrane Glycoproteins
TNF-Related Apoptosis-Inducing Ligand
TNFSF10 protein, human
Tumor Necrosis Factor-alpha