Promotion of U937 cell adhesion on polypropylene surfaces bearing phosphorylcholine functionalities

J Biomater Sci Polym Ed. 2004;15(11):1423-34. doi: 10.1163/1568562042368022.

Abstract

Phosphorylcholine (PC) groups were grafted onto ammonia plasma-treated biaxially-oriented polypropylene (BOPP) surfaces, via (a) reductive amination of phosphorylcholine glyceraldehyde and (b) a two-step procedure involving the chemical amplification of surface amine groups with tris(2-aminoethyl amine) and subsequent reductive amination of phosphorylcholine glyceraldehyde. The occurrence of grafting was ascertained by X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier-transform infrared (ATR-FT-IR) spectroscopy. The wettability of PC-modified surfaces was assessed by dynamic contact-angle measurements using the Wilhelmy plate method. Human U937 macrophages adhered and proliferated to a significantly larger extent on PC-modified surfaces, compared to unmodified or ammonia plasma-modified BOPP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonia / chemistry
  • Cell Adhesion
  • Humans
  • Macrophages / cytology*
  • Macrophages / metabolism*
  • Molecular Structure
  • Phosphorylcholine / analysis*
  • Phosphorylcholine / chemistry
  • Polypropylenes / chemistry*
  • Polypropylenes / metabolism*
  • Spectrum Analysis
  • U937 Cells

Substances

  • Polypropylenes
  • Phosphorylcholine
  • Ammonia