Abstract
CD8(+) cells from HIV-infected individuals showing the CD8(+) cell noncytotoxic antiviral response unexpectedly revealed mRNA for VCAM-1, a cell surface molecule found on endothelial cells. Uninfected subjects had undetectable levels of VCAM-1 mRNA in their CD8(+) cells. Flow cytometry analysis showed that up to 12% of the CD8(+) cells from HIV-positive individuals expressed VCAM-1 compared with 0.8% of the CD8(+) cells of HIV-negative individuals. Enrichment of the CD8(+)VCAM-1(+) cell population and subsequent coculture with CD4(+) cells acutely infected with HIV-1 showed that the VCAM-1(+)CD8(+) cells were able to suppress viral replication with 50% less input cells than the unseparated CD8(+) cell population. This study demonstrates, for the first time, the expression of VCAM-1 on CD8(+) cells. Moreover, the CD8(+)VCAM-1(+) cells show enhanced CD8(+) cell noncytotoxic antiviral response activity that could have clinical importance in HIV infection.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute Disease
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Adjuvants, Immunologic / biosynthesis*
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Adjuvants, Immunologic / genetics
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Adjuvants, Immunologic / physiology
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Anti-HIV Agents / metabolism*
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Antiviral Agents / biosynthesis*
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Antiviral Agents / genetics
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Antiviral Agents / physiology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism*
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CD8-Positive T-Lymphocytes / virology*
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Cell Membrane / immunology
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Cell Membrane / metabolism
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Cell Membrane / virology
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Cells, Cultured
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Coculture Techniques
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HIV Seronegativity / immunology
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HIV Seropositivity / genetics
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HIV Seropositivity / immunology
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HIV Seropositivity / metabolism
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HIV-1 / growth & development
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HIV-1 / immunology
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Humans
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Interleukin-15 / pharmacology
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / virology
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Lymphocyte Activation / genetics
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Lymphocyte Activation / immunology
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RNA, Messenger / biosynthesis
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T-Lymphocytes / immunology
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T-Lymphocytes / virology
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Up-Regulation / genetics
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Up-Regulation / immunology*
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Vascular Cell Adhesion Molecule-1 / biosynthesis*
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Vascular Cell Adhesion Molecule-1 / genetics
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Vascular Cell Adhesion Molecule-1 / physiology
Substances
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Adjuvants, Immunologic
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Anti-HIV Agents
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Antiviral Agents
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Interleukin-15
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RNA, Messenger
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Vascular Cell Adhesion Molecule-1