Abstract
Toxin-binding proteins of insect midgut epithelial cells are associated with insect resistance to Bacillus thuringiensis (Bt) Cry toxins. A 5378 nt cDNA encoding a 1717 amino acid putative midgut cadherin-like glycoprotein and candidate Cry1Ab toxin-binding protein was characterized from Ostrinia nubilalis. Intraspecific alignment of partial O. nubilalis cadherin gene sequences identified variance within proposed Cry1A toxin binding region 2 (TBR2), 1328IPLQTSILVVT[I/V] N1340, and flanking Cry1A toxin binding region 1 (TBR1), 861DIEIEIIDTNN871. DNA sequence and PCR-RFLP detected single nucleotide polymorphism between cadherin alleles, and pedigree analysis demonstrated Mendelian inheritance. A population sample from Mead, Nebraska showed allelic polymorphism. These assays may be useful for linkage mapping and field surveillance of wild populations and of O. nubilalis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Bacillus thuringiensis Toxins
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Bacterial Proteins / metabolism
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Bacterial Toxins / metabolism
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Base Sequence
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Binding Sites / genetics
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Cadherins / chemistry
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Cadherins / genetics*
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Cadherins / metabolism
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DNA, Complementary
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Endotoxins / metabolism
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Genetic Variation / physiology*
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Hemolysin Proteins
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Insect Proteins / chemistry
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Insect Proteins / genetics*
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Insect Proteins / metabolism
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Insecticide Resistance / genetics
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Molecular Sequence Data
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Moths / chemistry
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Moths / genetics*
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Phylogeny
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Polymorphism, Single Nucleotide
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Protein Binding
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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Cadherins
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DNA, Complementary
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Endotoxins
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Hemolysin Proteins
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Insect Proteins
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insecticidal crystal protein, Bacillus Thuringiensis