On increasing protein-crystallization throughput for X-ray diffraction studies

Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):123-9. doi: 10.1107/S0907444904027064. Epub 2005 Jan 19.

Abstract

Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction-size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1-6 protein-crystallization robot, have been implemented into a structural genomics project. The new two-step screening/optimization strategy yields diffraction-quality crystals directly from the screening process, reducing the need for further optimization. The ORYX modification involves the addition of extensions to the sample- and oil-delivery arms and software modifications that allow two plates to be set up simultaneously.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Protein Conformation
  • Proteins / chemistry*
  • X-Ray Diffraction

Substances

  • Proteins