Transforming growth factors type-beta and dexamethasone attenuate group II phospholipase A2 gene expression by interleukin-1 and forskolin in rat mesangial cells

FEBS Lett. 1992 Apr 20;301(2):190-4. doi: 10.1016/0014-5793(92)81245-h.

Abstract

Treatment of rat mesangial cells with interleukin-1 beta (IL-1 beta) and forskolin induced, in a synergistic fashion, the expression of group II phospholipase A2 (PLA2) mRNA, with subsequent increased synthesis and secretion of PLA2. In contrast, interleukin-6 did not increase PLA2 mRNA levels of PLA2 activity. Transforming growth factor (TGF) beta 1, TGF beta 2 and TGF beta 3 equipotently attenuated the IL-1 beta- and forskolin-induced elevation of PLA2 mRNA, as well as PLA2 synthesis and secretion. The glucocorticoid dexamethasone only partially suppressed the IL-1 beta- and forskolin-induced elevation of PLA2 mRNA, but totally inhibited PLA2 synthesis and secretion.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • Colforsin / pharmacology*
  • Dexamethasone / pharmacology*
  • Drug Synergism
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / enzymology*
  • Interleukin-1 / pharmacology*
  • Phospholipases A / genetics*
  • Phospholipases A / metabolism
  • Phospholipases A2
  • RNA, Messenger / metabolism
  • Rats
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Interleukin-1
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Colforsin
  • Dexamethasone
  • Phospholipases A
  • Phospholipases A2